Introduction: Resource optimization is an increasingly important goal in modern medicine and is strongly supported by the promotion of appropriateness in clinical practice. In cardiology, the high volume of laboratory test requests may lead to increased workload for healthcare professionals, higher costs, and a risk of unnecessary investigations. The aim of this study is to present the experience of the University Hospital of Ferrara (AOUFE) in promoting a more rational use of resources by improving the appropriateness of laboratory test prescribing, reducing variability while maintaining patient safety standards. Methods: A multidisciplinary working group composed of cardiologists and cardiology nurses was established. The team analyzed laboratory test prescribing patterns, identified areas of inappropriate use, and defined shared criteria based on scientific evidence and actual clinical needs. Standardized laboratory test panels were then defined for the most frequently performed interventional cardiac procedures, both before and after the intervention, while preserving clinicians’ autonomy to request additional tests in the presence of specific clinical conditions (e.g., post-procedural complications, symptoms, or electrocardiographic changes). The impact of the intervention was evaluated by comparing the number and type of laboratory tests ordered during the first six months before and after implementation of the new model. Results: Implementation of the pathway resulted in a significant reduction in unnecessary test requests (–20%), with a corresponding decrease in staff workload and a reduction in direct laboratory costs (–23.8%). No adverse events or diagnostic delays attributable to reduced prescribing were observed, confirming the maintenance of patient safety levels. Conclusions: A structured and shared approach between cardiologists and nurses can improve the appropriateness of laboratory test prescribing, contributing to more efficient resource utilization without compromising clinical safety. This model is easily transferable to other clinical settings and highlights the importance of regular review of clinical-care processes, as well as close physician–nurse collaboration, to achieve tangible improvements in quality, efficiency, and sustainability.